Validation of Molecular Markers of Barley Net Blotch Resistance Loci on Chromosome 3H for Marker-Assisted Selection

The most widespread and harmful disease of barley is net form blotch caused by the ascomycete Pyrenophora teres f. Drechsler (Ptt). A cost effective environmentally sustainable strategy for protection against Ptt to develop cultivars possessing genetic resistance. In previous GWA analysis, we identified SNP-markers associated with a resistance locus on chromosome 3H in interval 45.82–54.53 cM. These SNPs have been described previously literature be located within same region 3H. aim study was validate QTL markers controlling this KASP genotyping four F2 populations crosses between resistant cultivars, Morex, Fox, Zolo, accession, Local k-21578, susceptible cv. Gesine doubled haploid (DH) population Canadian Lake Shore (CLS)/Harrington. Eleven fifteen studied showed high efficacy (97.5–100%) co-segregation DH population, CLS/Harrington. Three these at 54.53 cM one 51.27 were two Morex Fox Gesine. are also close each other physical map (442,203,921–443,119,491 bp). Apparently, CLS, determined locus. Markers JHI-Hv50k-2016-166392 (47.1 cM, 112,536,071 bp), Clone ID 3255462_1 (51.63 363,531,898 3255462_2 363,531,871 bp) k-21578/Gesine. least loci exist 47.0–54.3 cM: 46.0–48.44 another 51.27–54.8 regions found harbor several genes involved important plant functions, including response signaling pathways. Allele-specific PCR developed based assay data tested six resistant, moderately genotypes. Four differentiate allele-specific will useful pyramiding QTLs marker-assisted selection.